This kit is used for in vitro qualitative nucleic acid detection of 2019-Novel Coronavirus (2019-nCoV), Influenza A and Influenza B simultaneously in respiratory specimens including oropharyngeal swabs and sputum. Primer sets and FAM labeled probes are designed for specific detection of both ORFlab and N genes of 2019-nCoV, VIC labeled probe for human RNase P gene as internal control, ROX labeled probe for M gene of Influenza A, CY5 labeled probe for NS gene of Influenza B.
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Components |
50 tests/kit |
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RT-PCR reaction buffer |
915 μL ×1 tube |
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RT-PCR enzyme mix |
75 μL ×1 tube |
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Positive control |
100 μL × 1 tube |
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Negative control |
100 μL × 1 tube |
Sensitivity：200 copies/ mL.
Specificity：Specificity：No cross reaction with SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63,Parainfluenza Virus, Human Adenovirus, Human Rhinovirus, Respiratory Syncytial Virus (A), Respiratory Syncytial Virus (B), Enterovirus A,Staphylococcus Aureus,Streptococcus Pneumonia,Klebsiella Pneumonia.
Precision： CV ≤ 5%.
Real-Time PCR System: Aehealth Diagenex AL, Molarray MA-6000, ABI 7500, ViiATM 7, QuantStudio 7 flex, Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM 5，SLAN-96S/96P