|
64T |
96T |
||
|
Component |
Dosage |
Component |
Dosage |
|
Reagent plate |
4 |
Lysis buffer B |
2 |
|
Lysis buffer B |
1 |
Lysis plate |
1 |
|
Plastic sleeve |
8 |
Wash 1 plate |
1 |
|
Protocol manual |
1 |
Wash 2 plate |
1 |
|
|
|
Elution plate |
1 |
|
|
|
Plastic sleeve |
1 |
|
|
|
Protocol manual |
1 |
96-well Round Hole Plate Preparation
64T Components to corresponding well plate as follows:
|
Well-Site |
10r7 |
2or8 |
3or9 |
4or10 |
5orll |
6orl2 |
|
Kit Component |
Lysis Buffer 600μL |
Wash Buffer1 500μL |
Wash Buffer2 500μL |
Blank |
Magnetic Beads 310μL |
Elute Buffer l00μL |
For 64T kit:
Carefully remove the heat sealing film on the reagent plate, and then add 200μL of sample and 20μL of lysis buffer B into the 1/7 column of the reagent plate.
For 96T kit:
Carefully remove the heat sealing film on the reagent plate, and then add 200μL of sample and 20μL of lysis buffer B into lysis plate.
Insert the reagent plate and plastic sleeve into the designated position of the instrument in order, and then click to run the "DNA/RNA" extraction program on the nucleic acid extractor.
At the end of the program, take out the plastic sleeve and discard it.
Take out the elution plate, and the eluent is extracted and stored in a new centrifuge tube for downstream experiments. If the downstream experiment cannot be carried out in time, the DNA sample can be stored at -20℃and the RNA sample can be stored at -80℃.