COVID-19 (ORF1ab, N, E)

RT-PCR kit for Novel Coronavirus 2019-nCoV

  • Size:50tests/kit
  • Components with different lot numbers cannot be used together.

Product Detail

Product Tags

Product Description

The novel coronaviruses belong to the B genus. COVID-19 is an acute respiratory infectious disease. People are generally susceptible. Currently, the patients infected by the novel coronavirus are the main source ofinfection; asymptomatic infected people can also be an infectious source. Based on the current epidemiological investigation, the incubation period is 1 to 14 days, mostly 3 to 7 days. The main manifestations include fever, fatigue and dry cough. Nasal congestion, runny nose, sore throat, myalgia and diarrhea are found in a few cases.

This kit is used for in vitro qualitative detection of novel coronavirus 2019-nCov in respiratory specimens including oropharyngeal swab, sputum, bronchoalveolar lavage fluid and nasopharyngeal swab. Primer sets and FAM labeled probe are designed for specific detection of ORFlab gene of 2019-nCov. VIC labeled probe for N gene of 2019-nCov. Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with CY5.

Kit Contents


50 tests/kit

RT-PCR Reaction Mix Reagent  

240μL ×1 tube

Enzyme Mix Reagent

72μL ×1 tube

2019-nCoV primer probe

48μL ×1 tube

Positive Control

200μL ×1 tube

Negative Control

200μL ×1 tube

Performance Index

Sensitivity: 200 copies/mL.

Specificity: No cross reaction with SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63 and HIN1, H3N2, H5N1, H7N9, Influenza B, Parainfluenza Virus (123), Rhinovirus(A,B,C), Adenovirus (1,2,3,4,5,7,55), Human interstitial pneumovirus, Human metapneumovirus, EBv, Measles virus, Human cytomegalic virus, Rota virus, Norovirus, Mumps virus, Varicella Zoster Virus, Mycoplasma pneumonia, Chlamydia pneumonia, Legionella, Bordetella pertussis, Haemophilus influenza, Staplhylococcus Aureus, Streptococcus Pneumonia, Streptococcus pyogenes, Klebsiella pneumonia, Tuberculous bacillus, Aspergillus fumigatus, Candida albicans, Candida glabrata, Cryptococcus neoformans.

Precision: CV ≤5%.

Applicable Instruments

Real-Time PCR System: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0. Bio-Rad CEX96 TouchTM SLAN-96S.SLAN-96P

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